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Evolutionary dynamics of Cucurbita argyrosperma from the Mesoamerican domestication center using SSR molecular markers PAB
Priori,Daniela; Villarreal,Daniel Zizumbo; Ek,Víctor Manuel de Jesús Canché; Limones-Briones,Verónica; Barbieri,Rosa Lía.
Abstract: The objective of this work was to evaluate the evolutionary dynamics of the wild-weedy-domestic gene pool of Cucurbita argyrosperma squash by estimating the levels of genetic diversity and gene flow in the putative area of its domestication. Nine populations were collected, and DNA was extracted from young leaves harvested separately from approximately 20 individuals in each population. The DNA fragments were amplified with eight pairs of SSR primers and separated by electrophoresis in 5% denaturing polyacrylamide gels. Genetic diversity and the amount of gene flow were estimated in the populations, and Bayesian grouping was used to determine the levels of gene infiltration and probability of ancestry. The ethnobotanical exploration indicated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cucurbitaceae; Genetic diversity; Microsatellite markers; Plant genetic resources; Squash.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000300287
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